Dating sequinning beading
The major advantage of this approach is the complete sequencing set can be performed in a single reaction, rather than the four needed with the labeled-primer approach.
This is accomplished by labelling each of the dideoxynucleotide chain-terminators with a separate fluorescent dye, which fluoresces at a different wavelength.
Sequencing results in a symbolic linear depiction known as a sequence which succinctly summarizes much of the atomic-level structure of the sequenced molecule.
This technique was also used to sequence the genome of James Watson recently.
The sequence of DNA encodes the necessary information for living things to survive and reproduce.
More genome data are now being produced by pyrosequencing than Sanger DNA sequencing.
Pyrosequencing has enabled rapid genome sequencing. Bacterial genomes can be sequenced in a single run with several times coverage with this technique.